The ExtraFree® Transport buffer is suitable for extraction-free PCR. So, there is no need for nucleic acid isolation from samples. Simply expose cells to ExtraFree® buffer for 15 minutes at ambient temperature or directly collect your swab in the buffer. Subsequently, incubate buffer containing samples for 5 minutes at 95 ºC to inactivate enzymes. The buffer is safe to handle; all reagents are non-toxic.